By Hans J. Vogel
Calcium-binding proteins play an enormous function in quite a few important organic tactics, starting from blood clotting and sign transduction in cells, to attaching proteins to membranes and serving as an critical resource of calcium. In Calcium-Binding Protocols-Volume 1: reports and Case stories and quantity 2: tools and Techniques-Hans Vogel and a panel of major researchers assessment the protein chemistry and behaviour of this important protein type, and supply a complete choice of confirmed experimental ideas for his or her examine either in vitro and in vivo. this primary quantity discusses the function of calcium in intracellular secondary messenger activation mechanisms, together with distinctive points of calcium chemistry and its usage in dairy proteins and blood clotting. distinct case experiences supply a wealth of useful information regarding protein purification and characterization innovations, X-ray crystallography, and particular calcium-binding proteins and their modes of motion. the second one significant other quantity, equipment and methods, makes a speciality of state of the art experimental tools for learning resolution constitution, balance, dynamics, calcium-binding houses, and organic job of calcium-binding proteins in general.
entire and hugely sensible, the 2 volumes of Calcium-Binding Protocols supply experimental and medical biologists with a bunch of complex experimental equipment that may be utilized effectively to the research of either present and newly chanced on participants of this significantly very important category of proteins.
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Additional resources for Calcium-Binding Protein Protocols: Volume 1: Reviews and Case Studies
70. , and Wuthrich, K. (1996) MOLMOL: a program for display and analysis of macromolecular structures. J. Mol. Graphics 14, 29–32. Calcium 21 2 Calcium Robert J. P. Williams 1. Introduction When one considers the chemistry of any element in the context of biological organisms, it is exceedingly important to observe it in relationship to the chemistries of all the other elements in the environment that are used, or even not used, by cells. One major concern is the limitation of the availability of the element concerned, in part because of the presence of other elements, which may have changed during the evolution of Earth and its organisms.
6A,B). 10. The Fixed Binding Constants for Calcium Ions in the Cytoplasm So far we have described proteins available for calcium binding K = 107 M–1, but most of them remain unbound in the resting state of the cell when [Ca2+] is less than 10–7 M. On excitation, the Ca2+ rises to 10–6 M. An amazing feature of the binding by intracytoplasmic proteins on excitation is therefore that, despite their variety, Table 6, the binding constants in the precise place where they are used, are virtually identical and close to 107 M–1.
1995) Structure of the Ca2+-free Gla domain sheds light on membrane binding of blood coagulation proteins. Nat. Struct. Biol. 2, 504–509. Erratum (1996) Nat. Struct. Biol. 3, 103. 5. , and Drakenberg, T. (1992) How an epidermal growth factor (EGF)-like domain binds calcium. High resolution NMR structure of the calcium form of the NH2-terminal EGF-like domain in coagulation factor X. J. Biol. Chem. 267, 19,642–19,649. 6. , and Engel, J. (1996) Extracellular calcium-binding proteins. Curr. Opin.
Calcium-Binding Protein Protocols: Volume 1: Reviews and Case Studies by Hans J. Vogel