By Melvin I. Simon, Jeremy Thorner, Scott D. Emr, John N. Abelson
The significantly acclaimed laboratory typical for greater than 40 years, Methods in Enzymology is among the so much hugely revered guides within the box of biochemistry. considering that 1955, each one quantity has been eagerly awaited, often consulted, and praised via researchers and reviewers alike. Now with greater than three hundred volumes (all of them nonetheless in print), the sequence comprises a lot fabric nonetheless suitable today--truly a vital book for researchers in all fields of lifestyles sciences
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Extra resources for Applications of Chimeric Genes and Hybrid Proteins - Part C: Protein-Protein Interactions and Genomics
Acad. Sci. A. 88, 9578 (1991). 328 Copyright© 2000by AcademicPress All rightsof reproductionin any formreserved. 00 [9,] L e x A - B A S E D TWO-HYBRID SYSTEMS 15 TABLE I COMPARISON OF PLASMIDS AND STRAINS FOR USE IN LexA Two-HYBRID SYSTEMSa Characteristic Basic bait plasmids Typical representative Yeast markers Bacterial markers Expression Alternative bait plasmids NLS enhanced Inducible expression COOH-terminal LexA Reporters I. Auxotrophic Typical representative Sensitivity regulation Yeast markers 2.
Golemis, I. Serebriiskii, J. Gyuris, and R. Brent, in "Current Protocols in Molecular Biology" (F. M. ), pp. 35. John Wiley & Sons, New York, 1997. 8 E. Golemis and I. Serebriiskii, in "Cells: A Laboratory Manual" (D. L. Spector, R. Goldman, and L. ). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 1997. 9 M. A. Watson, R. Buckholz, and M. P. Weiner, BioTechniques 21, 255 (1996). 10D. Gietz, A. St. Jean, R. A. Woods, and R. H. Schiestl, Nucleic Acids Res. 20, 1425 (1992). 11 j.
Cerevisiae L40 reporter strain, which contains two integrated reporters, the bacterial lacZ gene and the yeast HIS3 gene, which are both under the transcriptional control of lexA opera4 j. Estojak, R. Brent, and E. A. Golemis, Mol. Cell, Biol. 15, 5820 (1995). 5 D. M. Ruden, J. Ma, Y. Li, K. Wood, and M. Ptashne, Nature (London) 350, 250 (1991).  LexA-BASEDTWO-HYBRIDSYSTEMS System I 17 System II I constructLexA-baitproteinfusion) plasmid;transformyeast choose reporterplasmid~ .... sensitivityof auxo-"] .......
Applications of Chimeric Genes and Hybrid Proteins - Part C: Protein-Protein Interactions and Genomics by Melvin I. Simon, Jeremy Thorner, Scott D. Emr, John N. Abelson