By Yvonne A. Barnett
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Extra resources for Aging Methods and Protocols (Methods in Molecular Medicine)
The serum is stored at –20°C until use. Once thawed, serum is stored at 4°C for subsequent use; it should not be refrozen. Fibroblast Model for Cell Senescence Studies 33 Table 4 Components of Serum-Free Growth Medium Component Amount Supplier Cat. no. 9 g/L 25 mL/L 25 ng/mL 100 ng/mL 5 µg/mL 5 µM Trace 55 ng/mL Trace Gibco-BRL Sigma Sigma Sigma Sigma Sigma Sigma Gibco-BRL Gibco-BRL Sigma Sigma Sigma Sigma Pharmco 82-5006EA S5136 S5886 C7902 S5761 H9136 S2770 13247-010 13245-014 I6634 F8633 H9892 D4902 111000190CSGL aNot used in growth medium.
Brown, W. T. (1990) Genetic diseases of premature aging as models of senescence, in Annual Review of Gerontology and Geriatrics, vol. 10 (Cristofalo, V. J. and Lawton, M. ), Springer, New York, pp. 23–52. 30. Bodnar, A. , Holt, S. , Morin, G. , Harley, C. , Shay, J. , and Wright, W. E. (1998) Extension of life-span by introduction of telomerase into normal human cells. Science 279, 349–352. 31. Harley, C. , Shmookler Reis, R. , and Goldstein, S. (1982) Loss of repetitious DNA in proliferating somatic cells may be due to unequal recombination.
Pignolo, R. , Phillips, P. , and Cristofalo, V. J. (1992) Differential gene expression between young and senescent, quiescent WI-38 cells. Mech. Ageing Dev. 65, 239–255. 98. Praeger, F. C. and Gilchrest, B. A. (1986) Influence of increased extracellular calcium and donor age on density-dependent growth inhibition of human fibroblasts (42345) Proc. Soc. Exp. Biol. Med. 182, 315–321. 99. Praeger, F. C. and Cristofalo, V. J. (1986) The growth of WI-38 in a serum-free, growth factor-free, medium with elevated calcium concentrations.
Aging Methods and Protocols (Methods in Molecular Medicine) by Yvonne A. Barnett